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2 edition of Phenotypic characterisation of N. gonorrhoeae. found in the catalog.

Phenotypic characterisation of N. gonorrhoeae.

Maryanne Horgan

Phenotypic characterisation of N. gonorrhoeae.

by Maryanne Horgan

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Published by The Author] in [S.l .
Written in English


Edition Notes

Thesis (M. Sc. (Biomedical Sciences)) - University of Ulster, 1992.

The Physical Object
Paginationxii, 112p., tables, 5 plates :
Number of Pages112
ID Numbers
Open LibraryOL21162548M

  Several pertinent additional phenotypic and genotypic markers, for example, epidemiological markers, were also determined. CONCLUSIONS: The WHO N. gonorrhoeae reference strain panel was extensively characterized, which is crucial for the expansion of gonococcal AMR surveillance nationally and internationally. Phenotypic Characterization of Neisseria gonorrhoeae Isolated from Three Cases of Meningitis Meningitis is a rare complication of disseminated gonococ-cal infection (DGI). Although DGI has been estimated to occur in % [1] of all gonococcal infections, fewer than 30 cases have been reported in the past twenty years [].

The aims of the present study were to phenotypically and genetically characterise N gonorrhoeae and genetically characterise C trachomatis and M genitalium samples from women attending two sexual health clinics in Bissau, Guinea-Bissau and to define the bacterial populations, possible transmission chains and for N gonorrhoeae to define the presence and spread of antibiotic-resistant isolates from Cited by: 7.   Gonococcal isolates obtained between and in Slovenia (n = ) were investigated with Etest for susceptibility to cefixime, ceftriaxone, penicillin, ciprofloxacin, azithromycin, tetracycline, gentamicin and isolates were examined with N. gonorrhoeae multiantigen sequence typing for molecular epidemiology and sequencing of the major extended-spectrum Cited by:

  In this chapter, we describe a technique optimized for the phenotypic screening of N. gonorrhoeae FA and isogenic mutant strains. Inoculum size and culturing times have been adjusted for growth in chemically defined, protein-free Graver-Wade liquid medium in the well microtiter plate format employed by the PMs.   With the recent rise in the prevalence of fluoroquinolone resistance in southern Africa, there has been a regional move, initiated in South Africa () and Namibia (), to replace fluoroquinolones with single-dose oral cefixime. 12–14 This article describes the phenotypic and genetic characterization of the first two verified cases of N. gonorrhoeae resistant to oral third-generation Cited by:


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Phenotypic characterisation of N. gonorrhoeae by Maryanne Horgan Download PDF EPUB FB2

The authors thank the Institute of Environmental Science and Research (ESR) for providing N. gonorrhoeae isolates for this study. This work was supported by School of Food & Nutrition, Massey University (PR JAMA), Institute of Veterinary, Animal and Biomedical Sciences (IVABS), Massey University (RM NORSH), and The Hawke's Bay Medical Author: Norshuhaidah Jamaludin, Kristene Gedye, Julie Collins-Emerson, Jackie Benschop, Mary Nulsen.

Phenotypic Characterization of a copA Mutant of Neisseria gonorrhoeae Identifies a Link between Copper and Nitrosative Stress Karrera Y. Djoko,a Jessica A. Franiek,a Jennifer L. Edwards,b Megan L. Falsetta,c* Stephen P. Kidd,a* Adam J. Potter,a* Nathan H.

Chen, aMichael A. Apicella,c Michael P. Jennings,a* and Alastair G. McEwan Cited by: Phenotypic and genotypic characterization of Neisseria gonorrhoeae in parts of Italy: detection of a multiresistant cluster circulating in a heterosexual network - ScienceDirect.

JavaScript is disabled on your browser. Please enable JavaScript to use all the features on this page. Skip to Cited by: Conclusion There is an association between the phenotypic and genotypic character of N. gonorrhoeae isolates expressing reduced susceptibility to ceftriaxone in this study population.

Furthermore, the presence of important mosaic PBP2 that link to ceftriaxone treatment failure might be circulating among N. gonorrhoeae isolates in New : Mohd Jamaludin, Norshuhaidah bt. Genotypic characterization by pulsed-field gel electrophoresis, porB1b gene sequencing, and opa-typing revealed that these isolates were indistinguishable or closely related.

The results indicate that at least one PIP-negative N. gonorrhoeae strain is currently circulating in the Danish community, and this strain is indistinguishable from the Cited by: The eight N. gonorrhoeae WHO reference strains were phenotypically characterized by antibiogram, auxotype, serovar and prolyliminopeptidase screening; and genetically with regard to resistance.

Phenotypic and genotypic characterization of prolyliminopeptidase-negative Neisseria gonorrhoeae isolates in Denmark Article (PDF Available) in European Journal of Clinical Microbiology 24(4) Detection of N.

gonorrhoeae and Phenotypic Antimicrobial Susceptibility Determination. NAAT-based diagnosis of N. gonorrhoeae infection was performed using the Aptima Combo 2 nucleic acid amplification test kit (Hologic Inc., ) for N.

gonorrhoeae and Chlamydia trachomatis at Calgary Laboratory Services, Calgary, Alberta. The province of Alberta (population. New phenotypic and genotypic knowledge about N. gonorrhoeae has elucidated the bacterium’s effective ability to evade the host immune system and environmental threats like antimicrobials.

Gonorrhoea is here to stay. Objectives To describe the phenotypic and genetic characteristics of the first three cases of extended-spectrum cephalosporin (ESC) resistant Neisseria gonorrhoeae in South Africa which were associated, in one case, with a verified cefixime treatment failure.

Methods Three ESC resistant N. gonorrhoeae isolates were cultured from the urethral discharge of three men-who-have-sex-with-men (MSM. Phenotypic and genetic characterisation of bacterial sexually transmitted infections in Bissau, Guinea-Bissau, West Africa: A prospective cohort Available via license: CC BY-NC   Phenotypic and genotypic characterization of prolyliminopeptidase-negative Neisseria gonorrhoeae isolates in Denmark.

Fjeldsøe-Nielsen 1, Fredlund, H. et al. Phenotypic and genotypic characterization of prolyliminopeptidase-negative Neisseria gonorrhoeae isolates in Denmark. Eur J Clin Microbiol Infect ( Cited by: Genotypic and Phenotypic Characterization of Antimicrobial Resistance inNeisseria gonorrhoeae: a Cross-Sectional Study of Isolates Recovered from Routine Urine Cultures in a High- Incidence Setting.

Adam L. Bailey,aRobert F. Potter,bMeghan A. Wallace, Caitlin Johnson,aGautam Dantas,a,b,c,dCarey-Ann D. Burnhama,c,e,: Adam L. Bailey, Robert F. Potter, Meghan A. Wallace, Caitlin Johnson, Gautam Dantas, Carey-Ann D. Phenotypic and genotypic characterization of Neisseria gonorrhoeae in parts of Italy: detection of a multiresistant cluster circulating in a heterosexual network S.

Starnino1, B. Suligoi1, V. Regine1, N. Bilek2, P. Stefanelli1, and the Neisseria gonorrhoeae Italian. Objectives Identification of genetic targets specific to Neisseria gonorrhoeae for use in molecular detection methods has been a challenge.

The porA pseudogene in N gonorrhoeae has been commonly used but recently gonococcal isolates giving a negative result in these PCRs have been reported.

Here we describe the characterisation of two such gonococcal isolates received by the reference service. To determine the phenotypic and molecular characteristics of Neisseria gonorrhoeae isolates obtained between and in Slovenia. Gonococcal isolates obtained between and in Slovenia (naEuroS=aEuroS) were investigated with Etest for susceptibility to cefixime, ceftriaxone, penicillin, ciprofloxacin, azithromycin, tetracycline.

Conventional characterization of N. gonorrhoeae strains for epidemiological purposes is mostly based on phenotypic methods, which have some inherent limitations. In the present study sequence analysis of porB1b gene sequences was used for examination of the genetic relationships among N.

gonorrhoeae. 1 Characterisation of the OxyR regulon of Neisseria gonorrhoeae Kate L. Seib1†, Hsing-Ju Wu1§, Yogitha N.

Srikhanta1, Jennifer L. Edwards2, Megan L. Falsetta2, Amanda Hamilton1, Tina L. Maguire3, Sean M. Grimmond3, Michael A. Apicella2, Alastair G. McEwan1, Michael P. Jennings1* 1 School of Molecular and Microbial Sciences & Centre for Metals in Biology, The University of. These represented three known human-associated Neisseria species and included all the genomes available at the time of analysis for N.

polysaccharea (N = 45), all but one finished genomes of N. gonorrhoeae (N = 9) and representative genomes of the most common clonal complexes of N. meningitidis (N = 20) 43, with a preference for finished genomes when available. Because NAAT is the standard of care for diagnosis of N. gonorrhoeae, clinical isolates for detailed phenotypic and genotypic characterization are scarce.

New culture methods facilitated enhanced recovery of N. gonorrhoeae isolates in our laboratory, and subsequently, we characterized 64 patient isolates, including analysis of demography, geography, AST, and whole-genome sequencing. Consequently, precise phenotypic and genotypic characterisation of N.

gonorrhoeae isolates is crucial for gonorrhoea case management and for the development of .Studies using pathogenic Neisseria, N. gonorrhoeae, and N. meningitidis, have shown a complex series of events by which these human pathogens attach to, damage, and cross mucosal cells.

Phenotypic characterisation. One N. gonorrhoeae isolate from each culture-positive case (Case 1: H and Case 2: H) was sent to Public Health England’s national reference laboratory for characterisation.

Antimicrobial susceptibility testing was undertaken using minimum inhibitory concentration (MIC) gradient diffusion strips.